Hotspot

trnT-GGU-psbD

LSC · 31721-32964 · 1244 bp

Back to Persicaria

Pi 0.0528

MarkerSeek score 77.80

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1244
variable_sites: 249
pi: 0.0528
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGGAAGGTTTGTGGAATGGT	AAACTAGGCGCAAAGAACCA	50.0	45.0	58.0	58.0	1934-2380 bp	0.884615	87.1
trnT-GGU-psbD_p2	GGGAAGGTTTGTGGAATGGT	AACTAGGCGCAAAGAACCAA	50.0	45.0	58.0	58.0	1933-2379 bp	0.884615	87.1
trnT-GGU-psbD_p3	GGGAAGGTTTGTGGAATGGT	TCAGGGCCCCATAGTAACAA	50.0	50.0	58.0	58.0	1703-2149 bp	0.884615	87.1
trnT-GGU-psbD_p4	GGGAAGGTTTGTGGAATGGT	TTCAGGGCCCCATAGTAACA	50.0	50.0	58.0	58.0	1704-2150 bp	0.884615	87.1
trnT-GGU-psbD_p5	GGGAAGGTTTGTGGAATGGT	TCGAAATATAGCCGCTACGC	50.0	50.0	58.0	58.0	2249-2402 bp	0.769231	82.5