Hotspot

trnE-UUC-trnT-GGU

LSC · 30800-31648 · 849 bp

Back to Persicaria

Pi 0.0627

MarkerSeek score 78.10

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 849
variable_sites: 221
pi: 0.0627
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GGCTTATTGATGATGCACGC	GAACCGATGACTTACGCCTT	50.0	50.0	58.0	58.0	475-1030 bp	0.846154	85.5
trnE-UUC-trnT-GGU_p2	CCCAGGAACAACCAATTCCA	GAACCGATGACTTACGCCTT	50.0	50.0	58.0	58.0	930-1062 bp	0.730769	81.1
trnE-UUC-trnT-GGU_p3	ACCCAGGAACAACCAATTCC	GAACCGATGACTTACGCCTT	50.0	50.0	58.0	58.0	931-1063 bp	0.730769	81.1
trnE-UUC-trnT-GGU_p4	CCCAGGAACAACCAATTCCA	TAGAGAAGGCAGGGGATAGC	50.0	55.0	58.0	58.0	1425-1556 bp	0.692308	79.4
trnE-UUC-trnT-GGU_p5	ACCCAGGAACAACCAATTCC	TAGAGAAGGCAGGGGATAGC	50.0	55.0	58.0	58.0	1426-1557 bp	0.692308	79.4