Hotspot

trnD-GUC-trnY-GUA

LSC · 30162-30571 · 410 bp

Back to Persicaria

Pi 0.0641

MarkerSeek score 79.60

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 410
variable_sites: 105
pi: 0.0641
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	450-482 bp	1.0	86.3
trnD-GUC-trnY-GUA_p2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	449-481 bp	1.0	86.3
trnD-GUC-trnY-GUA_p3	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	471-503 bp	1.0	85.4
trnD-GUC-trnY-GUA_p4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	470-502 bp	1.0	85.2
trnD-GUC-trnY-GUA_p5	CGGTGCTCTGACCAATTGAA	CGGACTGTAAATTCGTTGGC	50.0	50.0	58.5	57.5	466-498 bp	1.0	84.0