Hotspot

petN-psbM

LSC · 28302-29147 · 846 bp

Back to Persicaria

Pi 0.0632

MarkerSeek score 75.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 846
variable_sites: 212
pi: 0.0632
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TTTGGTTAAGGCAAACGGGA	TGCGAGCCAATATCATCGAG	45.0	50.0	57.9	57.9	1594 bp	0.038462	40.4
petN-psbM_p2	TTTCCCCGGTTCAAATCTGG	TGCGAGCCAATATCATCGAG	50.0	50.0	58.1	57.9	1447 bp	0.038462	40.3
petN-psbM_p3	GCAAACGGGATTCAAGATGC	TGCGAGCCAATATCATCGAG	50.0	50.0	58.1	57.9	1584 bp	0.038462	40.3
petN-psbM_p4	CCCAAACCAAAAAGGGGGTA	TGCGAGCCAATATCATCGAG	50.0	50.0	57.9	57.9	1533 bp	0.038462	40.1
petN-psbM_p5	TCAATCGAAGGGGTGGATCT	TGCGAGCCAATATCATCGAG	50.0	50.0	58.1	57.9	1635 bp	0.038462	40.0