Hotspot

atpH-atpI

LSC · 13780-14415 · 636 bp

Back to Persicaria

Pi 0.0584

MarkerSeek score 80.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 636
variable_sites: 149
pi: 0.0584
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CCTCATCCTCAAATCAGCCC	TATCGTCCTTGGGTTCCCTT	55.0	50.0	58.0	58.0	1307-1581 bp	1.0	91.5
atpH-atpI_p2	CAGTGACCCAAGGAAACGAA	TATCGTCCTTGGGTTCCCTT	50.0	50.0	58.0	58.0	1614-1842 bp	0.923077	88.3
atpH-atpI_p3	ACGGAAGCGGCAGAAATAAT	TATCGTCCTTGGGTTCCCTT	45.0	50.0	58.0	58.0	947-1159 bp	0.692308	78.4
atpH-atpI_p4	TGCTAAAAGGCAAGCAGACA	TATCGTCCTTGGGTTCCCTT	45.0	50.0	58.0	58.0	1231-1433 bp	0.653846	74.4
atpH-atpI_p5	CTCATCCTCAAATCAGCCCC	TATCGTCCTTGGGTTCCCTT	55.0	50.0	58.0	58.0	1492 bp	0.038462	41.1