Hotspot

rps16-trnQ-UUG

LSC · 5974-7042 · 1069 bp

Back to Persicaria

Pi 0.0758
MarkerSeek score 85.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1069
variable_sites
328
pi
0.0758
unique_haplotype_count
23
primer_available
yes
alignment_reliability
0.99
markerseek_score
85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1073-1401 bp 1.0 90.3
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1084-1412 bp 1.0 89.8
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1077-1405 bp 1.0 85.5
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA TAAGGCAACGGGTTTTGGTC 45.0 50.0 58.1 58.7 1103-1431 bp 1.0 85.4
rps16-trnQ-UUG_p5 ATCATGTCCTTCAAGTCGCA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.2 58.1 1085-1413 bp 1.0 85.2