Hotspot

trnK-UUU-rps16

LSC · 4142-4863 · 722 bp

Back to Persicaria

Pi 0.0597

MarkerSeek score 79.20

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 722
variable_sites: 190
pi: 0.0597
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	GGTGTGGACGAATCCCTTAC	AAAAGGGTATGTTGCTGCCA	55.0	45.0	58.0	58.0	1333-1639 bp	1.0	91.8
trnK-UUU-rps16_p2	TCAGGATCAGTCGTGGTCTT	AAAAGGGTATGTTGCTGCCA	50.0	45.0	58.1	58.0	1368-1674 bp	1.0	91.3
trnK-UUU-rps16_p3	TTCAGGATCAGTCGTGGTCT	AAAAGGGTATGTTGCTGCCA	50.0	45.0	58.1	58.0	1369-1675 bp	1.0	91.3
trnK-UUU-rps16_p4	CCTTTCAGGATCAGTCGTGG	AAAAGGGTATGTTGCTGCCA	55.0	45.0	58.0	58.0	1470-1678 bp	0.846154	85.7
trnK-UUU-rps16_p5	CCTTTCAGGATCAGTCGTGG	TTTTCTTGAGCCGTACGAGG	55.0	50.0	58.0	57.9	1075-1281 bp	0.846154	85.1