Hotspot

ycf1

SSC · 128518-134082 · 5565 bp

Pi 0.00881

MarkerSeek score 44.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5565
variable_sites: 49
pi: 0.00881
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAAAGGACCAATGTCTGTCGA	AGAATGCTTGCCTAGAGTGT	47.6	45.0	58.0	56.5	232 bp	1.0	61.7
ycf1_p2	AGAAAGGACCAATGTCTGTCG	AGAATGCTTGCCTAGAGTGT	47.6	45.0	58.0	56.5	233 bp	1.0	61.7
ycf1_p3	AAAGGACCAATGTCTGTCGA	AGAATGCTTGCCTAGAGTGT	45.0	45.0	56.8	56.5	231 bp	1.0	60.3
ycf1_p4	GAAAGGACCAATGTCTGTCGA	AAGAATGCTTGCCTAGAGTGT	47.6	42.9	58.0	57.3	233 bp	1.0	59.7
ycf1_p5	AGAAAGGACCAATGTCTGTCG	AAGAATGCTTGCCTAGAGTGT	47.6	42.9	58.0	57.3	234 bp	1.0	59.7