Hotspot

rpl2

LSC · 87982-89481 · 1500 bp

Pi 0.00803

MarkerSeek score 50.50

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: LSC
length_bp: 1500
variable_sites: 12
pi: 0.00803
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	ATAATGGTAGATGCCCGGGA	GAATGGTTAAAGCGCCCAAC	50.0	50.0	58.0	58.0	2242-2255 bp	1.0	80.6
rpl2_p2	GGAATGCCCACGAATCTGAT	GAATGGTTAAAGCGCCCAAC	50.0	50.0	58.0	58.0	2602-2615 bp	1.0	80.5
rpl2_p3	ATAATGGTAGATGCCCGGGA	ACGGGGACATTCAATAAGGC	50.0	50.0	58.0	57.9	2175-2188 bp	1.0	80.3
rpl2_p4	GGAATGCCCACGAATCTGAT	ACGGGGACATTCAATAAGGC	50.0	50.0	58.0	57.9	2535-2548 bp	1.0	80.2
rpl2_p5	GGAATGCCCACGAATCTGAT	ATACTGGAACTCGTGGGGAA	50.0	50.0	58.0	58.1	2403-2416 bp	1.0	80.2