Hotspot

ccsA

SSC · 118997-119947 · 951 bp

Back to Persea

Pi 0.00736
MarkerSeek score 49.10
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
951
variable_sites
7
pi
0.00736
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 GCCCGCTGTTTAAAATGACC TTCTCGATGGCTTCTCTTGC 50.0 50.0 58.0 58.0 1885-1886 bp 1.0 80.6
ccsA_p2 TTATTTCGATGAAGCCGCCA TTCTCGATGGCTTCTCTTGC 45.0 50.0 58.0 58.0 1728-1729 bp 1.0 80.5
ccsA_p3 GCCCGCTGTTTAAAATGACC GCAGGAACGAGCTACGATAG 50.0 55.0 58.0 58.0 1981-1982 bp 1.0 80.4
ccsA_p4 GCCCGCTGTTTAAAATGACC CTCGATGGCTTCTCTTGCAT 50.0 50.0 58.0 58.0 1883-1884 bp 1.0 80.3
ccsA_p5 TTATTTCGATGAAGCCGCCA GCAGGAACGAGCTACGATAG 45.0 55.0 58.0 58.0 1824-1825 bp 1.0 80.2