Hotspot

rpl32-trnL-UAG

SSC · 117430-118828 · 1399 bp

Back to Persea

Pi 0.00943
MarkerSeek score 53.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1399
variable_sites
13
pi
0.00943
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TTTTTGTGCGACAAACAAGT TCCTAAGAGCAGCGTGTCTA 35.0 50.0 55.2 57.9 1458-1476 bp 1.0 58.1
rpl32-trnL-UAG_p2 TTTTTGTGCGACAAACAAGT AGCAGCGTGTCTACCAATTT 35.0 45.0 55.2 57.8 1451-1469 bp 1.0 57.6
rpl32-trnL-UAG_p3 TTTTTGTGCGACAAACAAGT CCTAAGAGCAGCGTGTCTAC 35.0 55.0 55.2 57.8 1457-1475 bp 1.0 57.5
rpl32-trnL-UAG_p4 TTTTTGTGCGACAAACAAGT CTAAGAGCAGCGTGTCTACC 35.0 55.0 55.2 57.8 1456-1474 bp 1.0 57.5
rpl32-trnL-UAG_p5 TTTTTGTGCGACAAACAAGT GAGCAGCGTGTCTACCAATT 35.0 50.0 55.2 58.3 1452-1470 bp 1.0 56.9