Hotspot

rpl2-rpl23

LSC · 89482-89516 · 35 bp

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Pi 0.0323
MarkerSeek score 73.60
Primer available yes
Label rpl2-rpl23

Feature Metadata

label_name
rpl2-rpl23
feature_type
igs
region
LSC
length_bp
35
variable_sites
1
pi
0.0323
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2-rpl23_p1 TCGCGGATTGGATTTCACTT CGCTTCAACCGGGTTATTCT 45.0 50.0 57.9 58.3 171-175 bp 1.0 79.3
rpl2-rpl23_p2 TTCGCGGATTGGATTTCACT CGCTTCAACCGGGTTATTCT 45.0 50.0 57.9 58.3 172-176 bp 1.0 79.3
rpl2-rpl23_p3 CGCGGATTGGATTTCACTTG CGCTTCAACCGGGTTATTCT 50.0 50.0 57.8 58.3 170-174 bp 1.0 78.8
rpl2-rpl23_p4 TCGCGGATTGGATTTCACTT ATCATTACGCTTCAACCGGG 45.0 50.0 57.9 58.3 178-182 bp 1.0 78.7
rpl2-rpl23_p5 TTCGCGGATTGGATTTCACT ATCATTACGCTTCAACCGGG 45.0 50.0 57.9 58.3 179-183 bp 1.0 78.7