Hotspot

petA-psbJ

LSC · 66485-67617 · 1133 bp

Back to Persea

Pi 0.0179
MarkerSeek score 67.00
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1133
variable_sites
20
pi
0.0179
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ACGTGTCCAAGGTTTGTTGT GGCTCATATTCCGGATTGGG 45.0 55.0 58.2 58.2 1278-1290 bp 1.0 79.6
petA-psbJ_p2 ACGTGTCCAAGGTTTGTTGT CGGCTCATATTCCGGATTGG 45.0 55.0 58.2 58.6 1279-1291 bp 1.0 76.5
petA-psbJ_p3 ACGTGTCCAAGGTTTGTTGT GCTCATATTCCGGATTGGGA 45.0 50.0 58.2 57.1 1277-1289 bp 1.0 74.1
petA-psbJ_p4 ACGTGTCCAAGGTTTGTTGT ATTCCGGATTGGGATCATCC 45.0 50.0 58.2 57.1 1271-1283 bp 1.0 73.9
petA-psbJ_p5 CGTGTCCAAGGTTTGTTGTT GGCTCATATTCCGGATTGGG 45.0 55.0 57.1 58.2 1277-1289 bp 1.0 73.5