Hotspot

trnH-GUG-psbA

LSC · 131-530 · 400 bp

Back to Persea

Pi 0.0447
MarkerSeek score 71.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
400
variable_sites
17
pi
0.0447
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CGAAGTTCCATCTACAAACGG 55.0 47.6 58.1 57.0 427-447 bp 1.0 69.8
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA CGAAGTTCCATCTACAAACGG 50.0 47.6 57.9 57.0 441-461 bp 1.0 69.6
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC CGAAGTTCCATCTACAAACGG 50.0 47.6 57.9 57.0 440-460 bp 1.0 69.6
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC CGAAGTTCCATCTACAAACGG 50.0 47.6 57.9 57.0 442-462 bp 1.0 69.5
trnH-GUG-psbA_p5 CGCATGGTGGATTCACAATC CGAAGTTCCATCTACAAACGG 50.0 47.6 57.5 57.0 456-476 bp 1.0 66.6