Hotspot

clpP

LSC · 66944-67597 · 654 bp

Back to Perotis

Pi 0.0178

MarkerSeek score 58.60

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 654
variable_sites: 23
pi: 0.0178
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GTTTAACCGTTGGCATGTCG	TATGCACCGAAAGCAATCGA	50.0	45.0	58.0	58.0	1372-1518 bp	1.0	82.6
clpP_p2	CTGAACGAGTCGCACATACA	TATGCACCGAAAGCAATCGA	50.0	45.0	58.0	58.0	1485-1631 bp	1.0	82.5
clpP_p3	CACCCTAGCACATGTTCCTC	TATGCACCGAAAGCAATCGA	55.0	45.0	58.0	58.0	1467-1613 bp	1.0	82.5
clpP_p4	CCGTTGGCATGTCGTATGTA	TATGCACCGAAAGCAATCGA	50.0	45.0	58.1	58.0	1366-1512 bp	1.0	82.4
clpP_p5	CCTAGCACATGTTCCTCGAC	TATGCACCGAAAGCAATCGA	55.0	45.0	58.1	58.0	1464-1610 bp	1.0	82.2