Hotspot

ndhF-rpl32

SSC · 102960-103869 · 910 bp

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Pi 0.0156

MarkerSeek score 65.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 910
variable_sites: 28
pi: 0.0156
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAAGTGGGAGAAGAGGGAT	TGACATCGAAGTACGTTTTT	55.0	35.0	58.1	53.2	990-996 bp	1.0	43.6
ndhF-rpl32_p2	GGAAGTGGGAGAAGAGGGAT	TTGACATCGAAGTACGTTTT	55.0	35.0	58.1	53.2	991-997 bp	1.0	43.6
ndhF-rpl32_p3	GGAAGTGGGAGAAGAGGGAT	TTTGACATCGAAGTACGTTT	55.0	35.0	58.1	53.2	992-998 bp	1.0	43.6
ndhF-rpl32_p4	GGAAGTGGGAGAAGAGGGAT	TTTTGACATCGAAGTACGTT	55.0	35.0	58.1	53.2	993-999 bp	1.0	43.6
ndhF-rpl32_p5	GGAAGTGGGAGAAGAGGGAT	TTTTTGACATCGAAGTACGT	55.0	35.0	58.1	53.2	994-1000 bp	1.0	43.6