Hotspot

infA-rps8

LSC · 75798-75876 · 79 bp

Back to Perotis

Pi 0.0274

MarkerSeek score 65.20

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 79
variable_sites: 4
pi: 0.0274
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	ACTTTTGCTTCTCTCGGGTT	CCTAAGGTTTTGGGCGGAAT	45.0	50.0	57.4	58.2	236-250 bp	1.0	79.0
infA-rps8_p2	ACTTTTGCTTCTCTCGGGTT	ATTCCTAAGGTTTTGGGCGG	45.0	50.0	57.4	58.2	239-253 bp	1.0	78.9
infA-rps8_p3	ACTTTTGCTTCTCTCGGGTT	TTTTGGGCGGAATGGGAATT	45.0	45.0	57.4	58.3	229-243 bp	1.0	77.4
infA-rps8_p4	ACTTTTGCTTCTCTCGGGTT	GGCGGAATGGGAATTGCTAT	45.0	50.0	57.4	58.4	224-238 bp	1.0	77.2
infA-rps8_p5	ACTTTTGCTTCTCTCGGGTT	TAAGGTTTTGGGCGGAATGG	45.0	50.0	57.4	58.4	234-248 bp	1.0	76.6