Hotspot

trnF-GAA-ndhJ

LSC · 47521-48062 · 542 bp

Back to Perotis

Pi 0.0194

MarkerSeek score 63.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 542
variable_sites: 20
pi: 0.0194
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	634-663 bp	1.0	81.4
trnF-GAA-ndhJ_p2	ATGCACAAGAAAGGGTCAGG	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	687-716 bp	1.0	80.9
trnF-GAA-ndhJ_p3	GCACAAGAAAGGGTCAGGAT	ATAGGCTGGCCCTTACGTAA	50.0	50.0	57.8	58.2	685-714 bp	1.0	79.9
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGCTGGCC	50.0	55.0	58.1	58.6	643-672 bp	1.0	78.2
trnF-GAA-ndhJ_p5	AATCCTCGTGTCACCAGTTC	ATAGGCTGGCCCTTACGTAA	50.0	50.0	57.5	58.2	637-666 bp	1.0	78.2