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atpI-atpH

LSC · 31064-31879 · 816 bp

Back to Perotis

Pi 0.00551

MarkerSeek score 64.80

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 816
variable_sites: 9
pi: 0.00551
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TAGCCGCAGCCTATATAGGT	CTCTACAGCTTGTCCTGCAG	50.0	55.0	57.5	58.0	964-974 bp	1.0	77.7
atpI-atpH_p2	ATTTTTGCAACGTTAGCCGC	CTCTACAGCTTGTCCTGCAG	45.0	55.0	58.6	58.0	977-987 bp	1.0	77.2
atpI-atpH_p3	TAGCCGCAGCCTATATAGGT	AGCCAATCCAGCAGCAATAA	50.0	45.0	57.5	57.9	901-911 bp	1.0	76.7
atpI-atpH_p4	TAGCCGCAGCCTATATAGGT	GCAGTACCTTGACCAACTCC	50.0	55.0	57.5	58.2	945-955 bp	1.0	76.3
atpI-atpH_p5	TAGCCGCAGCCTATATAGGT	AAGCAGCAGCAATTAGTGGA	50.0	45.0	57.5	57.8	878-888 bp	1.0	76.3