Hotspot

trnC-GCA-rpoB

LSC · 17929-19004 · 1076 bp

Back to Perotis

Pi 0.0255

MarkerSeek score 69.30

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1076
variable_sites: 51
pi: 0.0255
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1127-1160 bp	1.0	83.2
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1126-1159 bp	1.0	83.2
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	TCCCTCATTTCCATTCCGGA	50.0	50.0	58.1	58.4	1110-1143 bp	1.0	82.7
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	TTCCCTCATTTCCATTCCGG	50.0	50.0	58.1	57.6	1111-1144 bp	1.0	82.4
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	TCCGGGTATTGTGGACATTC	50.0	50.0	58.1	57.3	1128-1161 bp	1.0	80.2