Hotspot

psbM-petN

LSC · 16202-17038 · 837 bp

Back to Perotis

Pi 0.024
MarkerSeek score 66.50
Primer available yes
Label psbM-petN

Feature Metadata

label_name
psbM-petN
feature_type
igs
region
LSC
length_bp
837
variable_sites
34
pi
0.024
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-petN_p1 AAAACAGCCAGCCAAAATGA AGTATGGGGGAGAAGTGGAC 40.0 55.0 56.7 58.1 863-886 bp 1.0 75.1
psbM-petN_p2 AAAACAGCCAGCCAAAATGA GTATGGGGGAGAAGTGGACT 40.0 55.0 56.7 58.1 862-885 bp 1.0 75.1
psbM-petN_p3 AAAACAGCCAGCCAAAATGA TATGGGGGAGAAGTGGACTC 40.0 55.0 56.7 57.8 861-884 bp 1.0 74.6
psbM-petN_p4 AAAACAGCCAGCCAAAATGA GGGGGAGAAGTGGACTCTAG 40.0 60.0 56.7 58.2 858-881 bp 1.0 74.3
psbM-petN_p5 AACAGCCAGCCAAAATGATT GTATGGGGGAGAAGTGGACT 40.0 55.0 56.5 58.1 860-883 bp 1.0 73.2