Hotspot

trnD-GUC-psbM

LSC · 15357-16096 · 740 bp

Back to Perotis

Pi 0.0251

MarkerSeek score 70.80

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 740
variable_sites: 35
pi: 0.0251
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TCAATTGGTTAGAGCACCGC	TCATTTTGGCTGGCTGTTTT	50.0	40.0	58.6	56.7	895-948 bp	1.0	69.2
trnD-GUC-psbM_p2	TAGGTATGCCATACACCCCG	TCATTTTGGCTGGCTGTTTT	55.0	40.0	58.7	56.7	928-981 bp	1.0	68.2
trnD-GUC-psbM_p3	TCAATTGGTTAGAGCACCGC	AATCATTTTGGCTGGCTGTT	50.0	40.0	58.6	56.5	897-950 bp	1.0	67.3
trnD-GUC-psbM_p4	TCAATTGGTTAGAGCACCGC	ATCATTTTGGCTGGCTGTTT	50.0	40.0	58.6	56.5	896-949 bp	1.0	67.3
trnD-GUC-psbM_p5	TCAATTGGTTAGAGCACCGC	TCATTTTGGCTGGCTGTTTTT	50.0	38.1	58.6	57.4	895-948 bp	1.0	67.1