Hotspot

trnG-UCC-trnT-GGU

LSC · 13434-14267 · 834 bp

Back to Perotis

Pi 0.018

MarkerSeek score 65.40

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 834
variable_sites: 29
pi: 0.018
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	ACGAATCACACTTTTACCACT	CTACCGCTAAGGGAAAAGCC	38.1	55.0	55.5	58.3	948-973 bp	1.0	53.8
trnG-UCC-trnT-GGU_p2	AGAACGAATCACACTTTTACCAC	CTACCGCTAAGGGAAAAGCC	39.1	55.0	57.3	58.3	951-976 bp	1.0	52.7
trnG-UCC-trnT-GGU_p3	GAACGAATCACACTTTTACCACT	CTACCGCTAAGGGAAAAGCC	39.1	55.0	57.3	58.3	950-975 bp	1.0	52.7
trnG-UCC-trnT-GGU_p4	AACGAATCACACTTTTACCACT	CTACCGCTAAGGGAAAAGCC	36.4	55.0	56.2	58.3	949-974 bp	1.0	51.9
trnG-UCC-trnT-GGU_p5	ACGAATCACACTTTTACCACT	TCTACCGCTAAGGGAAAAGC	38.1	50.0	55.5	57.3	949-974 bp	1.0	51.0