Hotspot

ycf1

SSC · 123545-129001 · 5457 bp

Back to Periploca

Pi 0.0104

MarkerSeek score 48.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5457
variable_sites: 138
pi: 0.0104
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCAAAGCCACTTCGTTTCTC	AAAGCGCCGTTTATGAGGAT	50.0	45.0	58.0	58.0	1059-1065 bp	1.0	82.9
ycf1_p2	GCAAAGCCACTTCGTTTCTC	AAGCGCCGTTTATGAGGATT	50.0	45.0	58.0	58.0	1058-1064 bp	1.0	82.9
ycf1_p3	AGCGCGCATAGAACCTTTAA	GCCTTTTTCCAGATCCCGAT	45.0	50.0	58.0	57.9	503-509 bp	1.0	82.9
ycf1_p4	AGCGCGCATAGAACCTTTAA	AAAGCGCCGTTTATGAGGAT	45.0	45.0	58.0	58.0	1118-1124 bp	1.0	82.8
ycf1_p5	AGCGCGCATAGAACCTTTAA	AAGCGCCGTTTATGAGGATT	45.0	45.0	58.0	58.0	1117-1123 bp	1.0	82.8