Hotspot

trnN-GUU-ndhF

IRb · 108753-110403 · 1651 bp

Back to Periploca

Pi 0.00828

MarkerSeek score 54.90

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1651
variable_sites: 31
pi: 0.00828
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	CAATTGTGACCTCTCGGGAG	TGGGGGAACTAGAGGATTGG	55.0	55.0	58.0	58.1	2045-2206 bp	1.0	84.5
trnN-GUU-ndhF_p2	AGAATGATGGGGATAGCGGA	TGGGGGAACTAGAGGATTGG	50.0	55.0	58.0	58.1	2300-2461 bp	1.0	84.3
trnN-GUU-ndhF_p3	GCGAGCCCCTTTATTCTGAA	TGGGGGAACTAGAGGATTGG	50.0	55.0	58.0	58.1	1815-1976 bp	1.0	84.3
trnN-GUU-ndhF_p4	AAGCGAGCCCCTTTATTCTG	TGGGGGAACTAGAGGATTGG	50.0	55.0	58.0	58.1	1817-1978 bp	1.0	84.2
trnN-GUU-ndhF_p5	TTGTGACCTCTCGGGAGAAT	TGGGGGAACTAGAGGATTGG	50.0	55.0	58.1	58.1	2042-2203 bp	1.0	84.1