Hotspot

petA-psbJ

LSC · 63380-64087 · 708 bp

Back to Periploca

Pi 0.0106

MarkerSeek score 71.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 708
variable_sites: 18
pi: 0.0106
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.0	57.6	852-859 bp	1.0	81.4
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	835-842 bp	1.0	80.6
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	849-856 bp	1.0	80.3
petA-psbJ_p4	TTACGTGTCCAAGGCCTTTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.6	57.6	847-854 bp	1.0	79.0
petA-psbJ_p5	TACGTGTCCAAGGCCTTTTG	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.4	57.6	846-853 bp	1.0	78.6