Hotspot

ndhC-trnV-UAC

LSC · 50569-51670 · 1102 bp

Back to Periploca

Pi 0.0104
MarkerSeek score 57.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1102
variable_sites
28
pi
0.0104
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTAAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 31.8 55.0 53.9 58.7 733-1146 bp 1.0 46.1
ndhC-trnV-UAC_p2 TCATATTCGTAAAGCAGAAACA TTCGAGTCCGTATAGCCCTA 31.8 50.0 53.9 57.1 732-1145 bp 1.0 46.1
ndhC-trnV-UAC_p3 ATCATATTCGTAAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 30.4 55.0 54.5 58.7 734-1147 bp 1.0 46.1
ndhC-trnV-UAC_p4 TCATATTCGTAAAGCAGAAACAT GTTCGAGTCCGTATAGCCCT 30.4 55.0 54.5 58.7 733-1146 bp 1.0 46.1
ndhC-trnV-UAC_p5 CATATTCGTAAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 33.3 55.0 52.3 58.7 732-1145 bp 1.0 46.1