Hotspot

ycf3-trnS-GGA

LSC · 44462-44993 · 532 bp

Back to Periploca

Pi 0.0111
MarkerSeek score 63.10
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
532
variable_sites
14
pi
0.0111
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 CTCCCTTTCTCCCGAAGTTG CCCTCGGTAAACAAAAGCCT 55.0 50.0 57.9 58.1 685-714 bp 1.0 83.6
ycf3-trnS-GGA_p2 CTCCCTTTCTCCCGAAGTTG TTCCAATGCTACGCCTTGAA 55.0 45.0 57.9 57.8 656-685 bp 1.0 83.0
ycf3-trnS-GGA_p3 CCCTTTCTCCCGAAGTTGTC CCCTCGGTAAACAAAAGCCT 55.0 50.0 58.2 58.1 683-712 bp 1.0 82.9
ycf3-trnS-GGA_p4 CCCTTTCTCCCGAAGTTGTC TTCCAATGCTACGCCTTGAA 55.0 45.0 58.2 57.8 654-683 bp 1.0 82.3
ycf3-trnS-GGA_p5 CTCCCTTTCTCCCGAAGTTG ACCCTCGGTAAACAAAAGCC 55.0 50.0 57.9 58.4 686-715 bp 1.0 81.2