Hotspot

psaA-ycf3

LSC · 41701-42425 · 725 bp

Back to Periploca

Pi 0.0105

MarkerSeek score 58.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 725
variable_sites: 19
pi: 0.0105
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATCAAGCTGCCGAGTATTGG	50.0	50.0	57.9	58.0	903-921 bp	1.0	80.8
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	CGATCAAGCTGCCGAGTATT	50.0	50.0	57.9	58.1	905-923 bp	1.0	80.0
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	832-850 bp	1.0	80.0
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	836-854 bp	1.0	79.7
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	AGCTGCCGAGTATTGGAAAC	50.0	50.0	57.9	58.3	899-917 bp	1.0	79.0