Hotspot

petN-psbM

LSC · 28804-29995 · 1192 bp

Back to Periploca

Pi 0.00889

MarkerSeek score 66.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1192
variable_sites: 26
pi: 0.00889
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	TCATTCTAGTTCCTACCGCCT	50.0	47.6	58.0	58.0	1230-1288 bp	1.0	77.1
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	TCATTCTAGTTCCTACCGCCT	50.0	47.6	58.0	58.0	1228-1286 bp	1.0	77.1
petN-psbM_p3	ACTCGTAGTGTGGGGAAGAA	TCTAGTTCCTACCGCCTTTCT	50.0	47.6	58.0	57.9	1226-1284 bp	1.0	76.5
petN-psbM_p4	TCGTAGTGTGGGGAAGAAGT	TCTAGTTCCTACCGCCTTTCT	50.0	47.6	58.0	57.9	1224-1282 bp	1.0	76.5
petN-psbM_p5	CGTAGTGTGGGGAAGAAGTG	TCATTCTAGTTCCTACCGCCT	55.0	47.6	57.9	58.0	1227-1285 bp	1.0	76.5