Hotspot

trnS-GCU-trnG-UCC

LSC · 8265-8841 · 577 bp

Back to Periploca

Pi 0.017

MarkerSeek score 66.30

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 577
variable_sites: 23
pi: 0.017
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	651-737 bp	1.0	58.1
trnS-GCU-trnG-UCC_p2	CATCAAGGGAAGCGGAAAGA	ACGAATCACACTTTTACCACT	50.0	38.1	57.9	55.5	711-797 bp	1.0	57.2
trnS-GCU-trnG-UCC_p3	TCATCAAGGGAAGCGGAAAG	ACGAATCACACTTTTACCACT	50.0	38.1	57.9	55.5	712-798 bp	1.0	57.2
trnS-GCU-trnG-UCC_p4	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	45.0	36.4	58.0	56.2	652-738 bp	1.0	56.2
trnS-GCU-trnG-UCC_p5	CAAGGGAAGCGGAAAGAGAG	ACGAATCACACTTTTACCACT	55.0	38.1	58.3	55.5	708-794 bp	1.0	56.0