Hotspot

rps8

LSC · 79799-80203 · 405 bp

Back to Perilla

Pi 0.000823

MarkerSeek score 30.50

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 405
variable_sites: 1
pi: 0.000823
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	AGACAACTCACCCCCTTTCT	TTTGGGACGCCAAAAGTTTG	50.0	45.0	58.2	57.7	513-514 bp	1.0	76.5
rps8_p2	AGACAACTCACCCCCTTTCT	TTGGGACGCCAAAAGTTTGA	50.0	45.0	58.2	58.5	512-513 bp	1.0	74.7
rps8_p3	GACAACTCACCCCCTTTCTT	TTTGGGACGCCAAAAGTTTG	50.0	45.0	57.3	57.7	512-513 bp	1.0	72.6
rps8_p4	AGACAACTCACCCCCTTTCT	GGACGCCAAAAGTTTGAGTT	50.0	45.0	58.2	57.1	509-510 bp	1.0	72.0
rps8_p5	AGACAACTCACCCCCTTTCT	GGGACGCCAAAAGTTTGAGT	50.0	50.0	58.2	59.0	510-511 bp	1.0	71.1