Hotspot

rps3

LSC · 82297-82959 · 663 bp

Back to Perilla

Pi 0.00101

MarkerSeek score 33.20

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 663
variable_sites: 2
pi: 0.00101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	CCCCCTTTCCTTCTATCAATGG	ACAACTATCGCAAACCGTGA	50.0	45.0	58.2	57.8	754 bp	1.0	61.6
rps3_p2	CCCCCTTTCCTTCTATCAATGG	ACTATCGCAAACCGTGATGT	50.0	45.0	58.2	57.6	751 bp	1.0	59.9
rps3_p3	CCCCTTTCCTTCTATCAATGGA	ACAACTATCGCAAACCGTGA	45.5	45.0	57.2	57.8	753 bp	1.0	56.6
rps3_p4	CCCCTTTCCTTCTATCAATGGA	ACTATCGCAAACCGTGATGT	45.5	45.0	57.2	57.6	750 bp	1.0	54.8
rps3_p5	CCCCCTTTCCTTCTATCAATGG	CAACTATCGCAAACCGTGATG	50.0	47.6	58.2	58.0	753 bp	1.0	54.6