Hotspot

ndhF

SSC · 109338-111569 · 2232 bp

Back to Perilla

Pi 0.000597

MarkerSeek score 27.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 4
pi: 0.000597
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACTAAACAGGAACAAGGGCG	GCCCCATAGCCTTTCCTTTT	50.0	50.0	58.1	58.1	2953 bp	1.0	78.4
ndhF_p2	CGATCCACCGAAGAAGATCC	GCCCCATAGCCTTTCCTTTT	55.0	50.0	57.9	58.1	2935 bp	1.0	78.1
ndhF_p3	AAGGCGGATCCGAACAAAAT	GCCCCATAGCCTTTCCTTTT	45.0	50.0	58.2	58.1	2890 bp	1.0	77.9
ndhF_p4	ACAGCTTCCGAAATGAAGGG	GCCCCATAGCCTTTCCTTTT	50.0	50.0	58.2	58.1	2974 bp	1.0	77.8
ndhF_p5	CTAAACAGGAACAAGGGCGA	GCCCCATAGCCTTTCCTTTT	50.0	50.0	57.8	58.1	2952 bp	1.0	77.8