Hotspot

matK

LSC · 2018-3562 · 1545 bp

Back to Perilla

Pi 0.000647

MarkerSeek score 43.60

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1545
variable_sites: 3
pi: 0.000647
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TCAGAATAGAAATAGGGGAGATCC	AACCCCCGAATCTTCTACCT	41.7	50.0	56.8	57.7	1676-1703 bp	1.0	40.1
matK_p2	TCAGAATAGAAATAGGGGAGATCC	ACCCCCGAATCTTCTACCTT	41.7	50.0	56.8	57.7	1675-1702 bp	1.0	40.1
matK_p3	GGAGATCCCATTTTGATCTT	AACCCCCGAATCTTCTACCT	40.0	50.0	52.6	57.7	1660 bp	1.0	40.1
matK_p4	GGAGATCCCATTTTGATCTT	ACCCCCGAATCTTCTACCTT	40.0	50.0	52.6	57.7	1659 bp	1.0	40.1
matK_p5	GGAGATCCCATTTTGATCTTT	ACCCCCGAATCTTCTACCTT	38.1	50.0	53.5	57.7	1659 bp	1.0	40.1