Hotspot

atpF

LSC · 11833-13081 · 1249 bp

Back to Perilla

Pi 0.00107

MarkerSeek score 30.50

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1249
variable_sites: 4
pi: 0.00107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CCTACTTGAAGTACGGTGCC	TAACTTACGGGAAGGGCTGA	55.0	50.0	58.0	58.1	1690-1693 bp	1.0	79.9
atpF_p2	CCAGGGCGTTTATAACACGA	TAACTTACGGGAAGGGCTGA	50.0	50.0	58.0	58.1	1920-1923 bp	1.0	79.8
atpF_p3	CACCCGCCATTACTTCATCA	TAACTTACGGGAAGGGCTGA	50.0	50.0	58.0	58.1	1737-1740 bp	1.0	79.6
atpF_p4	TTAATTCTCGCTGACCACGG	TAACTTACGGGAAGGGCTGA	50.0	50.0	58.0	58.1	2085 bp	0.833333	72.8
atpF_p5	TTAATTCTCGCTGACCACGG	TGATTTCTGCTGCTTCCGTT	50.0	45.0	58.0	58.1	2351 bp	0.833333	72.5