Hotspot

ycf4-cemA

LSC · 60582-61240 · 659 bp

Back to Perilla

Pi 0.00152

MarkerSeek score 44.40

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 659
variable_sites: 3
pi: 0.00152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	GAAATCCACCAGGGCAGAAA	45.0	50.0	57.9	58.1	756 bp	1.0	79.1
ycf4-cemA_p2	CTTGCGCGTACCAATTGAAG	GAAATCCACCAGGGCAGAAA	50.0	50.0	58.1	58.1	758 bp	1.0	78.8
ycf4-cemA_p3	GAACAAAAAGCCGCGGAATT	GAAATCCACCAGGGCAGAAA	45.0	50.0	58.2	58.1	787 bp	1.0	77.9
ycf4-cemA_p4	TTTCTTGCGCGTACCAATTG	GAAATCCACCAGGGCAGAAA	45.0	50.0	57.7	58.1	761 bp	1.0	77.1
ycf4-cemA_p5	CCTATTTCTTGCGCGTACCA	GAAATCCACCAGGGCAGAAA	50.0	50.0	58.4	58.1	765 bp	1.0	76.9