Hotspot

atpB-rbcL

LSC · 54309-55110 · 802 bp

Back to Perilla

Pi 0.000831

MarkerSeek score 43.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 802
variable_sites: 2
pi: 0.000831
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CTGGCGGAAAAGCTACATCT	AACACCCGCTTTGAATCCAA	50.0	45.0	58.0	58.3	986 bp	1.0	77.5
atpB-rbcL_p2	GATGATACGCCCCAGGTTTT	AACACCCGCTTTGAATCCAA	50.0	45.0	57.9	58.3	946 bp	1.0	77.3
atpB-rbcL_p3	GGAAACCCCAGAACCAGAAG	AACACCCGCTTTGAATCCAA	55.0	45.0	58.1	58.3	913 bp	1.0	77.1
atpB-rbcL_p4	GATTTGGATGATACGCCCCA	AACACCCGCTTTGAATCCAA	50.0	45.0	57.7	58.3	952 bp	1.0	75.6
atpB-rbcL_p5	TGATTTGGATGATACGCCCC	AACACCCGCTTTGAATCCAA	50.0	45.0	57.7	58.3	953 bp	1.0	75.6