Hotspot

ycf3-trnS-GGA

LSC · 44016-44861 · 846 bp

Back to Perilla

Pi 0.000394

MarkerSeek score 43.60

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 846
variable_sites: 1
pi: 0.000394
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TGCCTCCTTTTCTCCTGAAG	AAGGTTCGGAAAGAGAGGGA	50.0	50.0	57.1	58.0	1044 bp	1.0	73.0
ycf3-trnS-GGA_p2	GCCTCCTTTTCTCCTGAAGT	AAGGTTCGGAAAGAGAGGGA	50.0	50.0	57.1	58.0	1043 bp	1.0	73.0
ycf3-trnS-GGA_p3	TGCCTCCTTTTCTCCTGAAG	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.1	58.1	1018 bp	1.0	72.5
ycf3-trnS-GGA_p4	GCCTCCTTTTCTCCTGAAGT	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.1	58.1	1017 bp	1.0	72.4
ycf3-trnS-GGA_p5	TGCCTCCTTTTCTCCTGAAG	TCGGAAAGAGAGGGATTCGA	50.0	50.0	57.1	57.9	1039 bp	1.0	72.1