Hotspot

psbC

LSC · 26868-28289 · 1422 bp

Back to Pericopsis

Pi 0.00141

MarkerSeek score 54.10

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 2
pi: 0.00141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	CTGCTCGACTCAACCCAATA	GAAGGTATTCGTGCGTGGAT	50.0	50.0	57.3	58.1	1519 bp	1.0	74.2
psbC_p2	CTGCTCGACTCAACCCAATA	GTTTCTCAAGAAATCCGCGC	50.0	50.0	57.3	58.1	1591 bp	1.0	73.8
psbC_p3	CTGCTCGACTCAACCCAATA	CGTTTCTCAAGAAATCCGCG	50.0	50.0	57.3	57.9	1592 bp	1.0	73.6
psbC_p4	CTGCTCGACTCAACCCAATA	GCAGCGGAAGATCCTGAATT	50.0	50.0	57.3	58.3	1573 bp	1.0	72.1
psbC_p5	TATGACCTGCTCGACTCAAC	GAAGGTATTCGTGCGTGGAT	50.0	50.0	57.1	58.1	1525 bp	1.0	72.1