Hotspot

psaC

SSC · 118798-119043 · 246 bp

Back to Pericopsis

Pi 0.00407

MarkerSeek score 44.50

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 1
pi: 0.00407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	GCGGGTTTTTGCCAGTAAAA	CTGGTAAATCATTGATTCATCTGGT	45.0	36.0	57.8	57.4	404 bp	1.0	40.2
psaC_p2	GCGGGTTTTTGCCAGTAAAA	TGGTAAATCATTGATTCATCTGGT	45.0	33.3	57.8	56.4	403 bp	1.0	40.2
psaC_p3	GCGGGTTTTTGCCAGTAAAA	TCTGGTAAATCATTGATTCATCTGG	45.0	36.0	57.8	57.2	405 bp	1.0	40.2
psaC_p4	ATTTTGAGCGCGGGTTTTTG	CTGGTAAATCATTGATTCATCTGGT	45.0	36.0	58.5	57.4	413 bp	1.0	40.2
psaC_p5	ATTTTGAGCGCGGGTTTTTG	TGGTAAATCATTGATTCATCTGGT	45.0	33.3	58.5	56.4	412 bp	1.0	40.2