Hotspot

ndhG-ndhI

SSC · 120409-120707 · 299 bp

Back to Pericopsis

Pi 0.0234

MarkerSeek score 66.00

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 299
variable_sites: 7
pi: 0.0234
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CAAGACCGGATCCCAGAAAG	AATCAAATTGCTTTGGGCCG	55.0	45.0	58.0	57.9	432-436 bp	1.0	80.3
ndhG-ndhI_p2	AAGACCGGATCCCAGAAAGA	AATCAAATTGCTTTGGGCCG	50.0	45.0	58.0	57.9	431-435 bp	1.0	80.1
ndhG-ndhI_p3	ATGTAGCGGTTCAGGCAAAT	AATCAAATTGCTTTGGGCCG	45.0	45.0	57.9	57.9	398-402 bp	1.0	79.6
ndhG-ndhI_p4	CAAGACCGGATCCCAGAAAG	TTTGGGCCGTTTACCAATGT	55.0	45.0	58.0	58.3	421-425 bp	1.0	78.7
ndhG-ndhI_p5	TACAAGACCGGATCCCAGAA	AATCAAATTGCTTTGGGCCG	50.0	45.0	57.8	57.9	434-438 bp	1.0	78.6