Hotspot

ndhD-psaC

SSC · 118679-118797 · 119 bp

Back to Pericopsis

Pi 0.0593

MarkerSeek score 83.20

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 119
variable_sites: 7
pi: 0.0593
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	CGGCTATCGGCAAAACTACA	AAACAACTCGAAGCATGGGT	50.0	45.0	58.4	58.0	202-203 bp	1.0	80.2
ndhD-psaC_p2	CGGCTATCGGCAAAACTACA	GGCATGAAACAACTCGAAGC	50.0	50.0	58.4	58.0	208-209 bp	1.0	80.0
ndhD-psaC_p3	CGGCTATCGGCAAAACTACA	CAGAGGACTGTGTTGGTTGT	50.0	50.0	58.4	57.7	283-284 bp	1.0	76.8
ndhD-psaC_p4	CGGCTATCGGCAAAACTACA	GTAAGAGGTGTGAATCCGCC	50.0	55.0	58.4	58.3	265-266 bp	1.0	76.8
ndhD-psaC_p5	CGGCTATCGGCAAAACTACA	TGTAAGAGGTGTGAATCCGC	50.0	50.0	58.4	57.6	266-267 bp	1.0	76.5