Hotspot

rpl32-trnL-UAG

SSC · 114792-115685 · 894 bp

Back to Pericopsis

Pi 0.00897
MarkerSeek score 55.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
894
variable_sites
8
pi
0.00897
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGGATATTGGACAGCATTGA TCCTAAGAGCAGCGTGTCTA 47.6 50.0 57.8 57.9 1013-1033 bp 1.0 70.0
rpl32-trnL-UAG_p2 GCGCAATCTATTTCTACGGG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 56.8 57.9 978-998 bp 1.0 70.0
rpl32-trnL-UAG_p3 AGCGCAATCTATTTCTACGGG TCCTAAGAGCAGCGTGTCTA 47.6 50.0 58.2 57.9 979-999 bp 1.0 69.9
rpl32-trnL-UAG_p4 GGGGATATTGGACAGCATTGA AGCAGCGTGTCTACCAATTT 47.6 45.0 57.8 57.8 1006-1026 bp 1.0 69.5
rpl32-trnL-UAG_p5 GCGCAATCTATTTCTACGGG AGCAGCGTGTCTACCAATTT 50.0 45.0 56.8 57.8 971-991 bp 1.0 69.5