Hotspot

rps11-rpl36

LSC · 80518-80952 · 435 bp

Back to Pericopsis

Pi 0.0189

MarkerSeek score 68.60

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 435
variable_sites: 8
pi: 0.0189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	ATGCTTACGCGAGCCAATAC	AAAATAAGGGCCTCCGTTCG	50.0	50.0	58.8	58.3	609-626 bp	1.0	74.2
rps11-rpl36_p2	CATGCTTACGCGAGCCAATA	AAAATAAGGGCCTCCGTTCG	50.0	50.0	58.8	58.3	610-627 bp	1.0	74.2
rps11-rpl36_p3	TTTTACGAGCATGCTTACGC	AAAATAAGGGCCTCCGTTCG	45.0	50.0	57.2	58.3	619-636 bp	1.0	74.0
rps11-rpl36_p4	ATGCTTACGCGAGCCAATAC	GCGGGGGCGAATTATAGTAA	50.0	50.0	58.8	57.5	556-573 bp	1.0	72.7
rps11-rpl36_p5	GCGAGCCAATACGTCCATTT	AAAATAAGGGCCTCCGTTCG	50.0	50.0	59.0	58.3	601-618 bp	1.0	72.6