Hotspot

atpA-trnR-UCU

LSC · 51954-52325 · 372 bp

Back to Pericopsis

Pi 0.00276

MarkerSeek score 50.60

Primer available yes

Label atpA-trnR-UCU

Feature Metadata

label_name: atpA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 372
variable_sites: 1
pi: 0.00276
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-trnR-UCU_p1	CATTCACTGGGGAAGCAGAA	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.8	57.3	486-521 bp	1.0	66.6
atpA-trnR-UCU_p2	AAGACATTCACTGGGGAAGC	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.8	57.3	490-525 bp	1.0	66.5
atpA-trnR-UCU_p3	GGAAGCAGAAGCCCTTTTGA	GGTTCAAATCCTATTGGACGC	50.0	47.6	58.4	57.3	476-511 bp	1.0	65.2
atpA-trnR-UCU_p4	ATTCACTGGGGAAGCAGAAG	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.5	57.3	485-520 bp	1.0	64.2
atpA-trnR-UCU_p5	GACATTCACTGGGGAAGCAG	GGTTCAAATCCTATTGGACGC	55.0	47.6	58.5	57.3	488-523 bp	1.0	63.9