Hotspot

trnS-UGA-psbC

LSC · 26682-26867 · 186 bp

Back to Pericopsis

Pi 0.0591

MarkerSeek score 82.40

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: LSC
length_bp: 186
variable_sites: 11
pi: 0.0591
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	GAGAGATGGCTGAGTGGTTG	TCATTTATGGCACGCAGGAA	55.0	45.0	58.0	57.9	381-383 bp	1.0	81.2
trnS-UGA-psbC_p2	GCTGAGTGGTTGATAGCTCC	TCATTTATGGCACGCAGGAA	55.0	45.0	58.1	57.9	373-375 bp	1.0	81.0
trnS-UGA-psbC_p3	GGCTGAGTGGTTGATAGCTC	TCATTTATGGCACGCAGGAA	55.0	45.0	58.1	57.9	374-376 bp	1.0	81.0
trnS-UGA-psbC_p4	GGTTGATAGCTCCGGTCTTG	TCATTTATGGCACGCAGGAA	55.0	45.0	58.1	57.9	366-368 bp	1.0	81.0
trnS-UGA-psbC_p5	GCTTTTGGAGAGATGGCTGA	TCATTTATGGCACGCAGGAA	50.0	45.0	57.9	57.9	388-390 bp	1.0	80.3