Hotspot

trnG-UCC-psbZ

LSC · 25215-26008 · 794 bp

Back to Pericopsis

Pi 0.0196

MarkerSeek score 68.40

Primer available yes

Label trnG-UCC-psbZ

Feature Metadata

label_name: trnG-UCC-psbZ
feature_type: igs
region: LSC
length_bp: 794
variable_sites: 15
pi: 0.0196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-psbZ_p1	CGTCTTCTCCTTGGCAAAGA	CTTCTCCTGATGGTTGGTCG	50.0	55.0	57.8	58.0	920-948 bp	1.0	82.2
trnG-UCC-psbZ_p2	CGTCTTCTCCTTGGCAAAGA	TTGCTTCTCCTGATGGTTGG	50.0	50.0	57.8	57.8	923-951 bp	1.0	80.7
trnG-UCC-psbZ_p3	CGTCTTCTCCTTGGCAAAGA	TCCTGATGGTTGGTCGAGTA	50.0	50.0	57.8	57.8	916-944 bp	1.0	80.5
trnG-UCC-psbZ_p4	CGTCTTCTCCTTGGCAAAGA	GCTTCTCCTGATGGTTGGTC	50.0	55.0	57.8	58.3	921-949 bp	1.0	80.3
trnG-UCC-psbZ_p5	CGTCTTCTCCTTGGCAAAGA	TTCTCCTGATGGTTGGTCGA	50.0	50.0	57.8	58.4	919-947 bp	1.0	79.4