Hotspot

ycf3-psaA

LSC · 18949-19850 · 902 bp

Back to Pericopsis

Pi 0.00342

MarkerSeek score 63.30

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: LSC
length_bp: 902
variable_sites: 3
pi: 0.00342
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	AGCCCTTACCCCTGGTAATT	TGCCCATTCCTCGAAAGAAG	50.0	50.0	58.0	57.9	1026-1050 bp	1.0	81.1
ycf3-psaA_p2	AGCCCTTACCCCTGGTAATT	GCCCATTCCTCGAAAGAAGT	50.0	50.0	58.0	57.9	1025-1049 bp	1.0	81.1
ycf3-psaA_p3	AGCTATAGCCCTTACCCCTG	TGCCCATTCCTCGAAAGAAG	55.0	50.0	58.0	57.9	1032-1056 bp	1.0	80.9
ycf3-psaA_p4	AGCTATAGCCCTTACCCCTG	GCCCATTCCTCGAAAGAAGT	55.0	50.0	58.0	57.9	1031-1055 bp	1.0	80.9
ycf3-psaA_p5	AGCCCTTACCCCTGGTAATT	GAGAAATGACCGGGTTTTGC	50.0	50.0	58.0	57.7	1043-1067 bp	1.0	79.2