Hotspot

trnM-CAU-trnV-UAC

LSC · 9342-9561 · 220 bp

Back to Pericopsis

Pi 0

MarkerSeek score 47.30

Primer available yes

Label trnM-CAU-trnV-UAC

Feature Metadata

label_name: trnM-CAU-trnV-UAC
feature_type: igs
region: LSC
length_bp: 220
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-trnV-UAC_p1	CCCGCCGTATGAAAGCAATA	CGTGTAAACGAGGTGCTCTA	50.0	50.0	58.4	57.4	298-303 bp	1.0	73.0
trnM-CAU-trnV-UAC_p2	CCGCCGTATGAAAGCAATAC	CGTGTAAACGAGGTGCTCTA	50.0	50.0	57.3	57.4	297-302 bp	1.0	71.0
trnM-CAU-trnV-UAC_p3	TTTGAACCAATGACTCCCGC	CGTGTAAACGAGGTGCTCTA	50.0	50.0	58.8	57.4	313-318 bp	1.0	70.2
trnM-CAU-trnV-UAC_p4	GACTCCCGCCGTATGAAAG	CGTGTAAACGAGGTGCTCTA	57.9	50.0	58.0	57.4	302-307 bp	1.0	68.1
trnM-CAU-trnV-UAC_p5	TTGAACCAATGACTCCCGC	CGTGTAAACGAGGTGCTCTA	52.6	50.0	58.1	57.4	312-317 bp	1.0	67.8